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A flow-through rearing system for ecophysiological studies of Pacific oyster Crassostrea gigas larvae ArchiMer
Rico Villa, Benjamin; Woerther, Patrice; Mingant, Christian; Le Piver, David; Pouvreau, Stephane; Hamon, Michel; Robert, Rene.
While literature is relatively abundant on adult shellfish, ecophysiological studies at the larval stage are scarce because of both technical difficulties and inadequate methodology. A tool dedicated to provide basic information for larval ecophysiology was accordingly developed. Two steps were followed: first a flow-through method of Crassostrea gigas larval culture was perfected during a set of experiments in which rearing systems and larval density were assessed. Then a continuous hydrobiological data recorder was adapted with modifications to comply with our experimental aim. SILO (Système d'Instrumentation des Larves en flux Ouvert) allowed the successive acquisition of hydrobiological parameters in ten 150 l larval tanks in which larvae were reared...
Tipo: Text Palavras-chave: Temperature; Ecophysiology; Bivalve larvae; Crassostrea gigas; Flow through.
Ano: 2008 URL: http://archimer.ifremer.fr/doc/2008/publication-4167.pdf
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Influence of food density and temperature on ingestion, growth and settlement of Pacific oyster larvae, Crassostrea gigas ArchiMer
Rico Villa, Benjamin; Pouvreau, Stephane; Robert, Rene.
Ingestion, growth and metamorphosis of Pacific oyster, Crassostrea gigas, larvae were studied under controlled conditions of food density and temperature using a combination of a flow-through rearing system and a hydrobiological monitoring device. In a first experiment larvae were exposed to three different phytoplankton densities (12, 20 and 40 cells μl-1) while in a second trial larvae were reared at five different temperatures (17, 22, 25, 27 and 32°C). Both food concentration and temperature significantly affected the larval physiology throughout the entire development from D-veliger to young spat. Larvae survived over a wide range of both environmental parameters with high survival at the end of experiments. The feeding functional response...
Tipo: Text Palavras-chave: Temperature; Food; Growth; Ecophysiology; Bivalve larvae; Crassostrea gigas.
Ano: 2009 URL: http://archimer.ifremer.fr/doc/2008/publication-4747.pdf
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Influence of phytoplankton diet mixtures on microalgae consumption, larval development and settlement of the Pacific oyster Crassostrea gigas (Thunberg) ArchiMer
Rico Villa, Benjamin; Le Coz, Jean-rene; Mingant, Christian; Robert, Rene.
Microalgae commonly used as feed for bivalves, Pavlova lutheri (P), Isochrisis affinis galbana (T) and Chaetoceros calcitrans forma pumilum (Cp), were fed to Pacific oyster Crassostrea gigas to assess their nutritional value for larval development and metamorphosis during two experiments. Monospecific, bispecific and trispecific diets were firstly evaluated during 3 weeks from D larvae to young postlarvae. Then bispecific diets, based on different T and Cp proportions, were assessed during a similar period. Concurrently, ingestion was studied through the whole larval and postlarval development for each diet and/or diet mixture. Because lipids are assumed to be a key nutrient for bivalves, biochemical analysis was undertaken on the second set of trials...
Tipo: Text Palavras-chave: Lipids; Grazing; Metamorphosis; Growth; Larvae; Crassostrea gigas.
Ano: 2006 URL: http://archimer.ifremer.fr/doc/2006/publication-1707.pdf
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Influence of sole and plurispecific diets on larval consumption and development of Pacific oyster Crassostrea gigas (Thunberg) ArchiMer
Robert, Rene; Rico Villa, Benjamin; Mingant, Christian.
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Ano: 2006 URL: http://archimer.ifremer.fr/doc/2006/publication-6695.pdf
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Les besoins écophysiologiques des larves d'huitres creuse Crassostrea gigas en conditions contrôlees : effet de la température, de la nourriture et modélisation de la croissance ArchiMer
Rico Villa, Benjamin.
Nowadays, spat production of Pacific oyster Crassostrea gigas relies on rearing techniques under controlled conditions in hatchery. In this context, an extensive research in aquaculture is necessary because larval development and metamorphosis are critical for hatchery management. Firstly, a diet consisted in a Haptophyceae: Isochrysis affinis galbana (clone T-ISO) and a Bacillariophyceae: Chaetoceros calcitrans forma pumilum demonstrated its efficiency to supply the nutritional needs throughout the whole larval rearing. Then, a flow-through rearing system was developed and coupled with a continuous hydrobiological data recorder to provide basic information for ecophysiology research through different trophic levels and/or temperature throughout larval...
Tipo: Text Palavras-chave: Crassostrea gigas; Larve; Huître; Croissance; Ecophysiologie; Microalgues; Température; Système d’élevage; Modélisation; Crassostrea gigas; Larvae; Oyster; Growth; Ecophysiology; Microalgae; Temperature; Rearing System; Modelling.
Ano: 2009 URL: http://archimer.ifremer.fr/doc/00012/12308/9102.pdf
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